treatment group mice  (MedChemExpress)

Journal: Frontiers in Immunology

Article Title: Identification and validation of BATF as a prognostic biomarker and regulator of immune cell infiltration in acute myeloid leukemia

doi: 10.3389/fimmu.2024.1429855

Figure Lengend Snippet: BATF expression is linked to the AML immune cell infiltration and immunotherapy efficacy. (A) ShBATF treatment and control C1498 cells were used for whole transcriptome RNA seq and subjected to KEGG enrichment analysis. (B) C57BL/6 or NOG mice (n = 8 per group) received intravenous injections of control or BATF knockdown C1498 cells, and their survival was monitored. (C–F) The proportion of T cells, NK cells, macrophages, and MDSC cells in the bone marrow of C1498 mouse models in the control group (n=4) and shBATF group (n=4). (G–J) The proportion of T cells, NK cells, macrophages, and MDSC cells in spleen of C1498 mouse models in the control group (n=4) and shBATF group (n=4). (K) Correlations between BATF expression and immune checkpoint expression. (L) Flow cytometry detection of PD-L1 expression in control cells and shBATF THP-1 cells. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, ns, nonsignificant.

Article Snippet: Treatment group C57BL/6 mice were treated with cytarabine (MCE) as an anti-cancer chemotherapy drug at 50 mg/kg via intraperitoneal injection for up to 2 weeks, while venetoclax (MCE) at 100 mg/kg via oral gavage daily for up to 1 weeks.

Techniques: Expressing, Control, RNA Sequencing Assay, Knockdown, Flow Cytometry

Journal: Chemical research in toxicology

Article Title: In Vivo Roles of Conjugation with Glutathione and O 6 -Alkylguanine DNA-Alkyltransferase in the Mutagenicity of the bis -Electrophiles 1,2-Dibromoethane and 1,2,3,4-Diepoxybutane in Mice

doi: 10.1021/tx4003534

Figure Lengend Snippet: Quantitative analysis of DNA adducts in livers of Big Blue® transgenic mice 6 h after treatment with dibromoethane (DBE), BSO/dibromoethane, or O6-BzGua/dibromoethane (A) and DEB, BSO/DEB, or O6-BzGua/DEB (B). O6-BzGua (80 mg/kg, ip) was administered 1 h prior to treatment with dibromoethane (30 mg/kg, ip) or DEB (25 mg/kg, ip), and BSO (8 mg/kg, ip) was administered 2 h prior to treatment with dibromoethane (30 mg/kg, ip) or DEB (25 mg/kg, ip).

Article Snippet: 12 Animals and Treatments Seventy male Big Blue® transgenic mice (five mice per group × 7 treatment groups × 2 time points), age 8 weeks (Agilent/Taconic) were housed in plastic cages (with bedding), according to U. S. National Institutes of Health guidelines.

Techniques: Transgenic Assay

Journal: Chemical research in toxicology

Article Title: In Vivo Roles of Conjugation with Glutathione and O 6 -Alkylguanine DNA-Alkyltransferase in the Mutagenicity of the bis -Electrophiles 1,2-Dibromoethane and 1,2,3,4-Diepoxybutane in Mice

doi: 10.1021/tx4003534

Figure Lengend Snippet: cII mutant frequencies in liver of Big Blue® transgenic mice at 6 (A, B) and 24 h (C, D) after treatment with vehicle, dibromoethane (DBE), BSO/dibromoethane, or O6-BzGua/dibromoethane (A, C) and vehicle, DEB, BSO/DEB, or O6-BzGua/DEB (B, D). O6-BzGua (80 mg/kg, ip) was administered 1 h prior to treatment with dibromoethane (30 mg/kg, ip) or DEB (25 mg/kg, ip) and BSO (8 mg/kg, ip) was administered 2 h prior to treatment with dibromoethane (30 mg/kg, ip) or DEB (25 mg/kg, ip). The statistical significance values were from compareisons with the cII mutant frequencies induced by dibromoethane or DEB and evaluated using Student’s t-test (shown in figure). In Part A, the relative mutations frequencies (compared to the highest value, with dibromethane treatment set at 100%) were 15% for vehicle, 42% for +BSO, and 88% for +O6-BzGua. In Part B, the relative mutations frequencies (compared to the highest value, with dibromethane treatment set at 100%) were 20% for vehicle, 43% for +BSO, and 88% for +O6-BzGua. In Part C, the relative mutations frequencies (compared to the highest value, with DEB treatment set at 100%) were 32% for vehicle, 57% for +BSO, and 67% for +O6-BzGua. In Part D, the relative mutations frequencies (compared to the highest value, with DEB treatment set at 100%) were 49% for vehicle, 68% for +BSO, and 80% for +O6-BzGua.

Article Snippet: 12 Animals and Treatments Seventy male Big Blue® transgenic mice (five mice per group × 7 treatment groups × 2 time points), age 8 weeks (Agilent/Taconic) were housed in plastic cages (with bedding), according to U. S. National Institutes of Health guidelines.

Techniques: Mutagenesis, Transgenic Assay

Journal: Chemical research in toxicology

Article Title: In Vivo Roles of Conjugation with Glutathione and O 6 -Alkylguanine DNA-Alkyltransferase in the Mutagenicity of the bis -Electrophiles 1,2-Dibromoethane and 1,2,3,4-Diepoxybutane in Mice

doi: 10.1021/tx4003534

Figure Lengend Snippet: Summary of Independent Mutations in the cII Gene of Livers of Big Blue® Transgenic Mice Treated with Vehicle, Dibromoethane, BSO/Dibromoethane, or O 6 -BzGua/ Dibromoethane

Article Snippet: 12 Animals and Treatments Seventy male Big Blue® transgenic mice (five mice per group × 7 treatment groups × 2 time points), age 8 weeks (Agilent/Taconic) were housed in plastic cages (with bedding), according to U. S. National Institutes of Health guidelines.

Techniques: Transgenic Assay, Mutagenesis

Journal: Chemical research in toxicology

Article Title: In Vivo Roles of Conjugation with Glutathione and O 6 -Alkylguanine DNA-Alkyltransferase in the Mutagenicity of the bis -Electrophiles 1,2-Dibromoethane and 1,2,3,4-Diepoxybutane in Mice

doi: 10.1021/tx4003534

Figure Lengend Snippet: Summary of Independent Mutations in the cII Gene of Livers of Big Blue® Transgenic Mice Treated with Vehicle, DEB, BSO/DEB, or O 6 -BzGua/DEB

Article Snippet: 12 Animals and Treatments Seventy male Big Blue® transgenic mice (five mice per group × 7 treatment groups × 2 time points), age 8 weeks (Agilent/Taconic) were housed in plastic cages (with bedding), according to U. S. National Institutes of Health guidelines.

Techniques: Transgenic Assay, Mutagenesis

Journal: Chemical research in toxicology

Article Title: In Vivo Roles of Conjugation with Glutathione and O 6 -Alkylguanine DNA-Alkyltransferase in the Mutagenicity of the bis -Electrophiles 1,2-Dibromoethane and 1,2,3,4-Diepoxybutane in Mice

doi: 10.1021/tx4003534

Figure Lengend Snippet: Relative independent mutations induced by vehicle (A), dibromoethane (DBE) (B), BSO/dibromoethane (C), or O6-BzGua/dibromoethane (D) in liver of Big Blue® transgenic mice. O6-BzGua (80 mg/kg, ip) was administered 1 h prior to treatment with dibromoethane (30 mg/kg, ip) and BSO (8 mg/kg, ip) was administered 2 h prior to treatment with dibromoethane (30 mg/kg, ip). Transition mutations (■); transversion mutations (□); frameshifts and others (diagonal shading). The P values are for comparison of the GC to AT transversions between Parts A and B (P <0.001) and between Parts B and C (P <0.001).

Article Snippet: 12 Animals and Treatments Seventy male Big Blue® transgenic mice (five mice per group × 7 treatment groups × 2 time points), age 8 weeks (Agilent/Taconic) were housed in plastic cages (with bedding), according to U. S. National Institutes of Health guidelines.

Techniques: Transgenic Assay

Journal: Chemical research in toxicology

Article Title: In Vivo Roles of Conjugation with Glutathione and O 6 -Alkylguanine DNA-Alkyltransferase in the Mutagenicity of the bis -Electrophiles 1,2-Dibromoethane and 1,2,3,4-Diepoxybutane in Mice

doi: 10.1021/tx4003534

Figure Lengend Snippet: Relative independent mutations induced by vehicle (A), DEB (B), BSO/DEB (C), or O6-BzGua/DEB (D) in livers of Big Blue® transgenic mice. O6-BzGua (80 mg/kg, ip) was administered 1 h prior to treatment with DEB (25 mg/kg, ip) and BSO (8 mg/kg, ip) was administered 2 h prior to treatment with DEB (25 mg/kg, ip). Transition mutations (■); transversion mutations (□); frameshifts and others (diagonal shading). The P values are for comparison of the AT to GC transversions between Parts A and B (P <0.0001), between Parts B ad C ((P <0.02) and between Parts B and C (P <0.05).

Article Snippet: 12 Animals and Treatments Seventy male Big Blue® transgenic mice (five mice per group × 7 treatment groups × 2 time points), age 8 weeks (Agilent/Taconic) were housed in plastic cages (with bedding), according to U. S. National Institutes of Health guidelines.

Techniques: Transgenic Assay